dna preparation streptococcus pyogenes cas9 (Addgene inc)
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dna preparation streptococcus pyogenes cas9
Dna Preparation Streptococcus Pyogenes Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+preparation+streptococcus+pyogenes+cas9/pMJ915+(Plasmid+%2369090)/pmc07398558-615-5-11
Average 93 stars, based on 37 article reviews
Dna Preparation Streptococcus Pyogenes Cas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dna+preparation+streptococcus+pyogenes+cas9/pMJ915+(Plasmid+%2369090)/pmc07398558-615-5-11
Average 93 stars, based on 37 article reviews
dna preparation streptococcus pyogenes cas9 - by Bioz Stars,
2026-09
93/100 stars
Images
1) Product Images from "The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells"
Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells
Journal: Molecular cell
doi: 10.1016/j.molcel.2020.06.014
Figure Legend Snippet: (A) Schematic of samples prepared for mass spectrometry. dCas9-BirA* programmed with the on-target gRNA, Cas9-BirA* programmed with the on-target guide, and dCas9-BirA* programmed with a NT sgRNA were incubated with a 10-fold molar excess of plasmid and then added to HSS containing the ARS and biotin. Biotinylated proteins were isolated with Streptavidin-coupled beads and identified through mass spectrometry.
Techniques Used: Mass Spectrometry, Incubation, Plasmid Preparation, Isolation
Figure Legend Snippet: (A) Time course of Cas9 RNPs programmed against a linear DNA substrate in a 1:2 molar ratio in buffer, HSS with ATP, or HSS with CIP and ATPγS.
Techniques Used:
Figure Legend Snippet: (A) HDR rates from amplicon-NGS sequencing of VEGFA 0, 1, 2, 3, 6, 9, 12, and 24 hours after electroporation of Cas9 RNPs in the presence of an HDR donor (N = 3 biological replicates). Data are represented as mean ± standard deviation. See also Figure S3E.
Techniques Used: Amplification, Sequencing, Electroporation, Standard Deviation
Figure Legend Snippet: KEY RESOURCES TABLE
Techniques Used: Purification, Recombinant, Amplification, Sequencing, Plasmid Preparation, Software
Related Articles
Mass Spectrometry:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Incubation:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Plasmid Preparation:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Isolation:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Amplification:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Sequencing:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Electroporation:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Standard Deviation:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Purification:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Recombinant:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. Software:Article Title: The histone chaperone FACT induces Cas9 multi-turnover behavior and modifies genome manipulation in human cells Article Snippet: K562 cells were maintained in RPMI (Gibco) supplemented with 10% fetal bovine serum, 1% sodium pyruvate, and 100 U/ml penicillin-streptomycin. |